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The resulting steriometric change of the molecular structure of the antibiotic prevents it from binding at its ribosome binding site
LB-Agar Low Salt
minus Arginine and without Glutamic acid
Tryptone and Yeast extract do not contain enough Mg2 for optimal adsorption
Cells are grown 1-2 days on pre-sporalation medium before transferring them to sporulation medium
Tetracycline HCl Size:25g The resulting steriometric change ofC22H24N1O8HCl = 480. 9 Tetracycline is a bacteriostatic antibioticum with activity against gram positive and gram negative bacteria. The antibiotic binds reversible to the 30S subunit of the ribosome, preventing the binding of aminoacyl transfer RNA and inhibiting protein synthesis and hence cell growth. Used as a selective marker for the transformation of plasmids encoding for tetracycline resistance (Tetr). CAS 64 75 5 Store dry at room temperature
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